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human visceral white preadipocytes  (ScienCell)

 
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    ScienCell human visceral white preadipocytes
    Human Visceral White Preadipocytes, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+visceral+white+preadipocytes/human+visceral+preadipocytes/pm37906396-47-0-6
    Average 90 stars, based on 1 article reviews
    human visceral white preadipocytes - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Transcriptomics profiling reveal the heterogeneity of white and brown adipocyte.
    Article Snippet: possession of large lipid droplets, a scarcity of mitochondria, and a lack of UCP1 expression for heat generation(Brestoff and Artis 2015).Brown adipocytes are characterized by their high quantity of small lipid droplets and numerous mitochondria.. Beige cells, which originate from Myf5 negative progenitor cells through de novo differentiation, are analogous to white adipocytes and are primarily found in the white adipose tissue region(Chu and Gawronska-Kozak 2017).. It is noteworthy that, apart from de novo transformation, white adipocytes possess the capability to undergo differentiation into beige adipocytes in response to cold and exercise stimuli, and this phenomenon is reversible.



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    Characterisation of IR-SVF cells. ( a ) Random images of cells were taken using a light microscope at ×200 magnification, of IR-SVF from passages 0–3. ( b ) The expression of preadipocyte markers CD10, CD73 and CD105, as well as the negative control CD45, was assessed using immunocytochemistry in both IR-SVF and commercial preadipocytes. Multiple random fields of view were captured at ×250 magnification. Primary antibodies for each cell type were identical; secondary antibodies for IR-SVF were RFP-tagged and for preadipocytes were GFP-tagged. ( c ) From the immunocytochemistry images, the percentage of cells (identified by nuclei) staining positive for each marker was quantified and expressed as a percentage. n = 1 donor for each cell population; scale bars = 100 µm. GFP, green-fluorescent protein; IR-SVF, stromal vascular fraction from irradiated tissue; RFP, red-fluorescent protein.

    Journal: Journal of Clinical Medicine

    Article Title: Stromal Vascular Fraction Cells from Individuals Who Have Previously Undergone Radiotherapy Retain Their Pro-Wound Healing Properties

    doi: 10.3390/jcm12052052

    Figure Lengend Snippet: Characterisation of IR-SVF cells. ( a ) Random images of cells were taken using a light microscope at ×200 magnification, of IR-SVF from passages 0–3. ( b ) The expression of preadipocyte markers CD10, CD73 and CD105, as well as the negative control CD45, was assessed using immunocytochemistry in both IR-SVF and commercial preadipocytes. Multiple random fields of view were captured at ×250 magnification. Primary antibodies for each cell type were identical; secondary antibodies for IR-SVF were RFP-tagged and for preadipocytes were GFP-tagged. ( c ) From the immunocytochemistry images, the percentage of cells (identified by nuclei) staining positive for each marker was quantified and expressed as a percentage. n = 1 donor for each cell population; scale bars = 100 µm. GFP, green-fluorescent protein; IR-SVF, stromal vascular fraction from irradiated tissue; RFP, red-fluorescent protein.

    Article Snippet: These were compared with commercially available human preadipocytes at passage 4 (Promocell).

    Techniques: Light Microscopy, Expressing, Negative Control, Immunocytochemistry, Staining, Marker, Irradiation

    CM from IR-SVF promotes DF scratch wound healing to a comparable degree as CM from commercial preadipocytes. IR-SVF and commercial preadipocytes were cultured in serum-free media for 24 h and CM collected. ( a ) Expression of chemokines FGF2, adiponectin and VEGF was assessed using ELISA. ( b ) The ability of CM to promote in vitro wound healing of DF monolayers was monitored for up to 48 h. ( c ) Representative images captured at ×40 magnification, scale bars = 500 um, white dotted line is wound edge. n = 4; ns = not significant; * p < 0.05. CM, conditioned media; DF, dermal fibroblast; ELISA, enzyme-linked immunosorbent assay; FGF2, fibroblast growth factor 2; IR-SVF, stromal vascular fraction from irradiated tissue; VEGF, vascular endothelial growth factor.

    Journal: Journal of Clinical Medicine

    Article Title: Stromal Vascular Fraction Cells from Individuals Who Have Previously Undergone Radiotherapy Retain Their Pro-Wound Healing Properties

    doi: 10.3390/jcm12052052

    Figure Lengend Snippet: CM from IR-SVF promotes DF scratch wound healing to a comparable degree as CM from commercial preadipocytes. IR-SVF and commercial preadipocytes were cultured in serum-free media for 24 h and CM collected. ( a ) Expression of chemokines FGF2, adiponectin and VEGF was assessed using ELISA. ( b ) The ability of CM to promote in vitro wound healing of DF monolayers was monitored for up to 48 h. ( c ) Representative images captured at ×40 magnification, scale bars = 500 um, white dotted line is wound edge. n = 4; ns = not significant; * p < 0.05. CM, conditioned media; DF, dermal fibroblast; ELISA, enzyme-linked immunosorbent assay; FGF2, fibroblast growth factor 2; IR-SVF, stromal vascular fraction from irradiated tissue; VEGF, vascular endothelial growth factor.

    Article Snippet: These were compared with commercially available human preadipocytes at passage 4 (Promocell).

    Techniques: Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay, In Vitro, Irradiation